Modulating Antiviral Immune Responses

Mice were treated intraperitoneally with 1 mg of anti-IFN-αR-1 clone MAR1–5A3 (Leinco, MO, USA), isotype immunoglobulin 1 (Leinco), 500 or 50 μg of anti-TNF-α clone XT3.11 (Bio X Cell, NH, USA) (42 (link)), or 250 μg of anti-IFN-γ clone XMG1.2 (Bio X Cell) (43 (link)). Mice were injected with 200 μg of poly I:C (Tocris, Bristol, UK).

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Shaabani N., Honke N., Nguyen N., Huang Z., Arimoto K.I., Lazar D., Loe T.K., Lang K.S., Prinz M., Knobeloch K.P., Zhang D.E, & Teijaro J.R. (2018). The probacterial effect of type I interferon signaling requires its own negative regulator USP18. Science immunology, 3(27), eaau2125.

Publication 2018

anti-TNF-α clone XT3.11 anti-IFN-γ clone XMG1.2 poly I:C

Cell Clone Ifn γ Immunoglobulin Isotype Mice Poly i c Tnf α

Corresponding Organization : Heinrich Heine University Düsseldorf

Other organizations : University of California, San Diego, University of Freiburg

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Variable analysis

independent variables

1 mg of anti-IFN-αR-1 clone MAR1–5A3
Isotype immunoglobulin 1
500 or 50 μg of anti-TNF-α clone XT3.11
250 μg of anti-IFN-γ clone XMG1.2
200 μg of poly I:C

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

Positive control: Isotype immunoglobulin 1
Negative control: Not specified

Annotations

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