Genome Sequencing of Pepper-Associated Bacterium

P. ogarae RDP1 was isolated from the rhizosphere of pepper (Capsicum annuum) as previously described [26 (link)]. Total DNA was extracted using the Realpure Genomic DNA extraction Kit (Durviz, Spain) and sequenced by paired-end Illumina MiSeq 2×150 at Parque Científico de Madrid (Spain). Reads were quality-filtered using Trimmomatic software v0.38 [42 (link)]. The genome of P. ogarae RDP1 was assembled using SPAdes v3.14.1 software [43 (link)], read error correction and careful parameters. Prokka v1.14.6 software [44 (link)] was used to annotate the genome, using the genetic code number 11 and default parameters. The genome sequence has been deposited in the NCBI and it is publicly available under the BioProject accession number PRJNA697838.

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Garrido-Sanz D., Redondo-Nieto M., Martin M, & Rivilla R. (2021). Comparative genomics of the Pseudomonas corrugata subgroup reveals high species diversity and allows the description of Pseudomonas ogarae sp. nov.. Microbial Genomics, 7(6), 000593.

Publication 2021

MiSeq 2×150

Capsicum annuum Genetic code Genome Pepper Rhizosphere

Corresponding Organization :

Other organizations : University of Lausanne, Autonomous University of Madrid

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Variable analysis

independent variables

Isolation source: rhizosphere of pepper (Capsicum annuum)

dependent variables

Genome assembly and annotation of P. ogarae RDP1

control variables

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