Whole-Exome Sequencing of ADHD Cohort

Whole-exome sequencing was carried out as previously described [38 (link)]. Paired-end, indexed libraries for Illumina sequencing were prepared from the DNAs of the 50 ADHD and age- and gender-matched non-ADHD children. No other selection criteria were used. Library enrichment for WES was conducted using SureSelect Human All Exon v7 from Agilent Technologies (Santa Clara, CA, USA) [39 (link)]. Enriched samples were sequenced using an Illumina NovaSeq 6000 platform (San Diego, CA, USA) [40 (link)]. Mean coverage of the sequences was 50× on target. The study design, laboratory experiment and bioinformatics pipeline based on the modified protocol of Tombacz et al. is presented in Figure 1 [41 (link)].