Transcriptome Analysis of Tree Peony

Tree peony (Paeonia rockii) with the same genetic origin was grown in the wild tree peony germplasm repository at Yangling, Shaanxi Province, China. They were identified by Professor Li-xin Niu from Northwest A&F University. The voucher specimens of Paeonia rockii were deposited into the Herbarium of the National Oil Peony Engineering Technology Research Center, China. Different tissues including the root, stem, leaf, calyx, petal, stamen, pistil, and developing seeds (20, 40, 60, 80, and 100 days after flowering) were collected for transcript level analysis. All the samples were immediately frozen in liquid nitrogen and stored at -80 ℃ for further studies. Arabidopsis thaliana (ecotype Columbia-0) and Nicotiana benthamiana plants used for transformation were grown in growth chambers at 21/25 ℃ (day/night) with a 16 h light/8 h dark.

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Xie L., Hu J., Yan Z., Li X., Wei S., Xu R., Yang W., Gu H, & Zhang Q. (2023). Tree peony transcription factor PrWRI1 enhances seed oil accumulation. BMC Plant Biology, 23, 127.

Publication 2023

Arabidopsis thaliana Calyx Ecotype Frozen Genetic Leaf Light Nicotiana Nitrogen Paeonia Peony Pistil Plants Root Seeds Stem Tissues Tree

Corresponding Organization :

Other organizations : Zhengzhou University, Chengdu Research Base of Giant Panda Breeding, Chinese Academy of Agricultural Engineering, Northwest A&F University

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Variable analysis

independent variables

Tissue type (root, stem, leaf, calyx, petal, stamen, pistil, and developing seeds at 20, 40, 60, 80, and 100 days after flowering)

dependent variables

Transcript level

control variables

Growing conditions for Arabidopsis thaliana (ecotype Columbia-0) and Nicotiana benthamiana plants used for transformation (21/25 °C day/night, 16 h light/8 h dark)

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

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