Immunohistochemical Localization of Calcium-Binding Protein in Harbor Porpoise Brain

To reveal neurons containing the calcium-binding protein CB, we used the CB38a anti-CB rabbit polyclonal antibody from Swant (CB38a; RRID AB_10000340) at a dilution of 1:10,000. The pattern of staining within the neurons of the basal forebrain, diencephalon, and pons of the harbor porpoise was similar to that seen in other mammals (Gritti et al., 2003 (link); Bunce et al., 2013 (link); Bhagwandin et al., 2013 (link)).

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Dell L.A., Patzke N., Spocter M.A., Siegel J.M, & Manger P.R. (2016). Organization of the Sleep-Related Neural Systems in the Brain of the Harbour Porpoise (Phocoena phocoena). The Journal of comparative neurology, 524(10), 1999-2017.

Publication 2016

CB38a

Anti antibody Basal forebrain Calcium binding protein Diencephalon Dilution Harbor porpoise Mammals Neurons Pons Rabbit Seen

Corresponding Organization : University of the Witwatersrand

Other organizations : Des Moines University, VA Sepulveda Ambulatory Care Center, University of California, Los Angeles

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Variable analysis

independent variables

Use of the CB38a anti-CB rabbit polyclonal antibody from Swant

dependent variables

Pattern of staining within the neurons of the basal forebrain, diencephalon, and pons of the harbor porpoise

control variables

Dilution of the CB38a antibody at 1:10,000

controls

The pattern of staining observed was similar to that seen in other mammals, as referenced in the following publications: Gritti et al., 2003; Bunce et al., 2013; Bhagwandin et al., 2013.

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