SDS-PAGE and immunoblot analyses were performed as previously described (Shoji et al., 2011 (link)). The blotted membranes were treated with anti-TfsA and anti-TfsB antisera (Sakakibara et al., 2007 (link)).
The glycoproteins in SDS-PAGE gels were stained using the Pro-Q Emerald 300 fluorescent stain (Invitrogen). After staining with Pro-Q Emerald 300, total protein staining was performed with SYPRO Ruby (Invitrogen).
The glycoproteins in SDS-PAGE gels were stained using the Pro-Q Emerald 300 fluorescent stain (Invitrogen). After staining with Pro-Q Emerald 300, total protein staining was performed with SYPRO Ruby (Invitrogen).
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