For SPEM mouse models, 350 mg/kg of L635 (n = 3) or 350 mg/kg of DMP-777 (n = 3) was administered to female or male C57BL/6 mice at 8 weeks of age, as previously described, [27 (link), 28 (link)] This animal experiment followed protocols approved by the Institutional Animal Care and Use Committees (IACUC) of Vanderbilt University.
Female BALB/c nu/nu mice at 6–8 weeks of age (Orient Bio., Sungnam, Korea) were used to establish tumor xenograft models. For the tumorigenicity assay, we subcutaneously injected empty vector-transfected (n = 8) or stable miR-30a-overexpressing (n = 8) SNU-601 cells into the right flank of the mice; a total of 1 × 107 cells in 200 μl of medium was injected into each mouse. Tumor size was measured twice every week using callipers, and the tumor volume was calculated by length (L) × width (W) × height (H). On day 63, all mice were sacrificed, and the tumor mass was pathologically assessed by haematoxylin and eosin (H&E) staining. This animal experiment was approved by the IACUC of the Clinical Research Institute at Seoul National University Hospital [14–0183-C1A0(1)].
Seesupplementary materials and methods for more information.
Female BALB/c nu/nu mice at 6–8 weeks of age (Orient Bio., Sungnam, Korea) were used to establish tumor xenograft models. For the tumorigenicity assay, we subcutaneously injected empty vector-transfected (n = 8) or stable miR-30a-overexpressing (n = 8) SNU-601 cells into the right flank of the mice; a total of 1 × 107 cells in 200 μl of medium was injected into each mouse. Tumor size was measured twice every week using callipers, and the tumor volume was calculated by length (L) × width (W) × height (H). On day 63, all mice were sacrificed, and the tumor mass was pathologically assessed by haematoxylin and eosin (H&E) staining. This animal experiment was approved by the IACUC of the Clinical Research Institute at Seoul National University Hospital [14–0183-C1A0(1)].
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