FRAP Analysis of Cellular Dynamics

FRAP experiments and analysis were performed as described before (Scotter et al, 2014 (link)) in a Leica SP5 inverted confocal laser microscope using 63X objective (NA 1.4). Cells were plated on 24‐well plates and cultured with phenol red‐free DMEM (Gibco, 31053028).

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Pérez‐Berlanga M., Wiersma V.I., Zbinden A., De Vos L., Wagner U., Foglieni C., Mallona I., Betz K.M., Cléry A., Weber J., Guo Z., Rigort R., de Rossi P., Manglunia R., Tantardini E., Sahadevan S., Stach O., Hruska‐Plochan M., Allain F.H., Paganetti P, & Polymenidou M. (2023). Loss of TDP‐43 oligomerization or RNA binding elicits distinct aggregation patterns. The EMBO Journal, 42(17), e111719.

Publication 2023

SP5 inverted confocal laser microscope DMEM

Cells Confocal microscope

Corresponding Organization :

Other organizations : University of Zurich, University Hospital of Zurich, Laboratory for Biomedical Neurosciences, Ente Ospedaliero Cantonale, ETH Zurich

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Fluorescence recovery after photobleaching (FRAP)

control variables

Cell culture in phenol red-free DMEM (Gibco, 31053028)
Microscope setup: Leica SP5 inverted confocal laser microscope using 63X objective (NA 1.4)

controls

Positive control: Not mentioned
Negative control: Not mentioned

Annotations

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