FAEE-Induced Acute Pancreatitis in Mice

To establish FAEE-induced AP (FAEE-AP), adult CD1 mice received two intraperitoneal injections of ethanol (1.35 g/kg) and POA (150 mg/kg) at 1 h intervals. 200 µL normal saline was injected immediately prior to ethanol/POA injections to avoid potential local damage by ethanol to peritoneal organs at the injection site. Control adult CD1 mice received either saline, ethanol (1.35 g/kg) or POA (150 mg/kg). In treatment groups, mice also received 10 mg/kg 4-MP or 30 mg/kg 3-BCP simultaneously with the first injection of ethanol/POA. Animals were sacrificed at 24 h after the first injection. Histological assessment of damage was performed after H&E staining of fixed pancreatic slices (5 µm thickness); 10 random fields per slide from all animal groups were graded by two independent, blinded observers according to severity and extent of oedema, inflammatory cell infiltration and acinar necrosis as previously described.15 (link) Further details of FAEE-AP model characterisation are included in online supplementary materials and methods.