HNSC-Secretome Impact on SCI Recovery

This research was a true experimental study. The sample size was calculated using the Lemeshow formula (n = 5 rats), with correction factors of 20%. The rats were randomly grouped into the following three groups: the treatment group (five experimental rats had SCI with HNSC-secretome), the control group (five experimental rats had SCI without HNSC-secretome), and the normal group (five experimental rats did not have SCI and did not get HNSC-secretome). The treatment group received a 30 µL HNSC-secretome intrathecal injection at the T10 level three days after the SCI and laminectomy [8 (link),14 (link)]. All groups were replicated five times. The authors observed the study over 28 days [15 (link)]. The independent variable of the study is HNSC-secretome, where as the dependent variables are neuropathic pain, locomotor function, TNF-α, F2-Isoprostane, MMP-9, TGF-β, and BDNF.

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Semita I.N., Utomo D.N., Suroto H., Sudiana I.K, & Gandi P. (2023). The mechanism of human neural stem cell secretomes improves neuropathic pain and locomotor function in spinal cord injury rat models: through antioxidant, anti-inflammatory, anti-matrix degradation, and neurotrophic activities. The Korean Journal of Pain, 36(1), 72-83.

Publication 2023

F2 isoprostane Intrathecal injection Laminectomy Mmp 9 Neuropathic pain Rats Secretome Tgf β Tnf α

Corresponding Organization :

Other organizations : Airlangga University, Universitas Jember

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Variable analysis

independent variables

HNSC-secretome

dependent variables

Neuropathic pain
Locomotor function
TNF-α
F2-Isoprostane
MMP-9
TGF-β

control variables

SCI without HNSC-secretome
No SCI and no HNSC-secretome

controls

Positive control: SCI with HNSC-secretome
Negative control: SCI without HNSC-secretome
Normal control: No SCI and no HNSC-secretome

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