Evaluating Leukemia and Solid Tumor Cell Lines

Murine leukemia cell lines were isolated from mice that had developed leukemias carrying different chimeric FGFR1 genes as described previously [18 (link)] and their immunophenotype was confirmed using standard flow cytometry analysis. The KG1 cell line was shown to carry the FGFR1OP2-FGFR1 rearrangement demonstrating the identity of this cell line. Human lung H1581 and H2228 and breast MDA-MB-134VI and T47D cancer cells were purchased from ATCC (passage number < 15). All cell lines were cultured in RPMI (Invitrogen) with 5% FBS (Hyclone), at 37°C in 10% CO₂. For drug treatments, 40,000 leukemia cells/well or 5,000 solid tumor cells/well were seeded in 96-well plates and incubated overnight, then treated with the either DMSO (control) or the drugs indicated in the results section at concentrations defined by the experiments. Cell viability was determined using Cell Titer-Glo luminescence cell viability kits (Promega) and a SpectraMax^® M5e (Molecular Probe) luminescence plate reader [18 (link)].