The ability of inorganic silver nanoparticles to form stable colloids when it reacts with plant extract is referred to as the colloidal stability of green synthesized nanoparticles. Colloidal stability test was performed according to the standard protocol.21 (link) MA-AgNPs were added to five different solutions namely NaCl salt solution (1.8%, w/v), PBS buffer (pH 7.4), Dulbecco’s Modified Eagle’s Medium (DMEM), CM (complete culture medium) and deionized water. Absorbance was measured by UV-Spectrophotometer (UV1601, Shimadzu) between wavelengths ranging from 100–900 nm. Further, the on-shelf stability in deionized water was assessed at RT for up to 9 days. Absorbance was periodically measured by a UV-Spectrophotometer (UV1601, Shimadzu) between wavelengths ranging from 100–900 nm.
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Colloidal Stability of Green Synthesized Nanoparticles
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Other organizations : Temasek Life Sciences Laboratory, National University of Singapore
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Variable analysis
independent variables
- Type of solution added to MA-AgNPs (NaCl salt solution, PBS buffer, DMEM, CM, deionized water)
- Absorbance of MA-AgNPs measured by UV-Spectrophotometer between wavelengths ranging from 100–900 nm
- On-shelf stability of MA-AgNPs in deionized water over time (up to 9 days)
- UV-Spectrophotometer model (UV1601, Shimadzu)
- Wavelength range (100–900 nm)
- Positive control: Not mentioned
- Negative control: Not mentioned
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