Total, cytoplasmic, and nuclear proteins of cells were extracted and assessed by Western blot analysis with antibodies using a standard protocol described previously [26 (link),27 (link)]. We used the following antibodies: anti-PLCɛ (Santa Cruz); anti-PCNA, anti-Cyclin D1, anti-β-actin, anti-p-AMPKα, anti-total-AMPKα, ACLY, ACACA, SCD1 (Cell Signaling Technology), anti-SREBP-1, anti-H3 (Abcam), and FASN (Sigma).
Protocol detail
Protein extraction and Western blot analysis
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anti-PLCɛ
anti-PCNA
anti-Cyclin D1
anti-β-actin
anti-p-AMPKα
anti-total-AMPKα
ACACA
anti-SREBP-1
anti-H3
Acaca
Actin
Antibodies
Cells
Cyclin d1
Cytoplasmic
Fasn
Nuclear proteins
Pcna
Srebp 1
Western blot analysis
Corresponding Organization :
Other organizations : First Affiliated Hospital of Chongqing Medical University, Chongqing Medical University, Chongqing Cancer Hospital, Chongqing University, Jiamusi University
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Variable analysis
independent variables
- Total, cytoplasmic, and nuclear proteins of cells
- Protein expression levels measured by Western blot analysis
- Standard protocol described previously [26,27]
- Antibodies used: anti-PLCɛ, anti-PCNA, anti-Cyclin D1, anti-β-actin, anti-p-AMPKα, anti-total-AMPKα, ACLY, ACACA, SCD1, anti-SREBP-1, anti-H3, FASN
- Positive controls not explicitly mentioned
- Negative controls not explicitly mentioned
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