Total RNA was extracted using RNA Iso-plus reagent (Takara Bio). Ultraviolet spectrophotometer was used for measuring RNA concentration and purity. The reverse transcription PCR process was performed using PrimeScript RT Reagent Kit according to the manufacturer’s protocol.16 (link) Primers were designed by Primer 5.0 (Primer-E, Ltd., Plymouth, United Kingdom), (Table 2), and the reverse primer for miR-122 and U6 PCR used as internal control was the Uni-miR qPCR Primer offered by the kit.17 (link) The reverse transcription reaction contained 25 μL of total RNA and miRNA PrimeScript RT Enzyme Mix, and the 20 μL real-time PCR (RT-PCR) was performed in the ABI PRISM 7300 Fast Real-Time PCR System (Ambion, Foster City, CA, USA), with the conditions: 95°C for 5 minutes, 95°C for 10 seconds, and 40 cycles at 60°C for 20 seconds. The reaction for Wnt1- and EMT-related genes was performed under conditions of 95°C for 10 seconds, 95°C for 30 seconds, and 40 cycles at 60°C for 30 seconds. The products were separated by electrophoresis on 0.2% agarose gels. The results of RT-PCR were expressed as 2−ΔΔCt.