Western Blot Analysis of Apoptosis Markers

Cells were collected and western blot analyses were performed as previously described.^{33 (link)}β-actin was used as the loading control. The primary antibodies used for western blotting included: rabbit anti-cleaved PARP (#9544, Cell Signaling, Beverly, MA, USA), anti-H2AX (#9718, Cell Signaling), anti-Akt1 (#9514, SBA), anti-Akt2 (#8715, SBA), anti-Akt3 (#4059, Cell Signaling), anti-pAkt (#4060, Cell Signaling) and anti-β-actin (#4970, Cell Signaling).

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Tan P.X., Du S.S., Ren C., Yao Q.W., Zheng R., Li R, & Yuan Y.W. (2014). MicroRNA-207 enhances radiation-induced apoptosis by directly targeting akt3 in cochlea hair cells. Cell Death & Disease, 5(10), e1433-.

Publication 2014

rabbit anti-cleaved PARP anti-H2AX anti-Akt3 anti-pAkt anti-β-actin

Actin Akt1 Akt2 Akt3 Antibodies Cells Rabbit Western blot analyses

Corresponding Organization :

Other organizations : Southern Medical University, Nanfang Hospital

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Cleaved PARP

control variables

β-actin (used as loading control)

controls

Positive control: Not specified
Negative control: Not specified

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