Resin Embedding and TEM Imaging of Fixed Kidney Samples

Modified PLP-fixed kidneys were additionally post-fixed for 1 hour at room temperature with 1% osmium tetroxide in cacodylate buffer and were exposed to a graded series of ethanol solutions to 100% for dehydration. They were then infiltrated with Epon resin (Ted Pella, Redding, CA), sectioned, and examined in a JEOL JEM 1011 transmission electron microscope (JEOL, Peabody, MA) as previously described^{59 (link)}. Images were collected using an AMT digital imaging system (Advanced Microscopy Techniques, Danvers, MA).

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Tsuji K., Păunescu T.G., Suleiman H., Xie D., Mamuya F.A., Miner J.H, & Lu H.A. (2017). Re-characterization of the Glomerulopathy in CD2AP Deficient Mice by High-Resolution Helium Ion Scanning Microscopy. Scientific Reports, 7, 8321.

Publication 2017

Epon resin JEM 1011 transmission electron microscope AMT digital imaging system

Buffer Cacodylate Dehydration Epon Ethanol Kidneys Microscopy Osmium tetroxide Resin Transmission electron microscope

Corresponding Organization :

Other organizations : Center for Systems Biology, Harvard University, Massachusetts General Hospital, Washington University in St. Louis

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Variable analysis

independent variables

Post-fixing with 1% osmium tetroxide in cacodylate buffer
Dehydration with graded series of ethanol solutions to 100%
Infiltration with Epon resin

dependent variables

Ultrastructural features of the fixed and processed kidney samples
Images collected using an AMT digital imaging system

control variables

Modified PLP-fixed kidneys
Sectioning and examination in a JEOL JEM 1011 transmission electron microscope

controls

No positive or negative controls were explicitly mentioned in the provided information.

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