For the GBS of the melon collection, leaf tissue was taken from 44 of the 51 accessions, listed in Table 1. Total genomic DNA isolation was performed as described by Gur et al. [37 (link)] using the GenEluteTM Plant Genomic DNA miniprep kit (Sigma, St. Louis, MO, USA). The quality of the DNA was analyzed with an ND-1000 spectrophotometer (Nanodrop Technologies, Wilmington, DE) and by electrophoresis on agarose gel. The concentration of DNA was estimated using a Qubit® 2.0 Fluorometer (Life Technologies, City, Singapore) and a Qubit® dsDNA BR Assay Kit (Life Technologies, Eugene, OR, USA).
Moing A., Allwood J.W., Aharoni A., Baker J., Beale M.H., Ben-Dor S., Biais B., Brigante F., Burger Y., Deborde C., Erban A., Faigenboim A., Gur A., Goodacre R., Hansen T.H., Jacob D., Katzir N., Kopka J., Lewinsohn E., Maucourt M., Meir S., Miller S., Mumm R., Oren E., Paris H.S., Rogachev I., Rolin D., Saar U., Schjoerring J.K., Tadmor Y., Tzuri G., de Vos R.C., Ward J.L., Yeselson E., Hall R.D, & Schaffer A.A. (2020). Comparative Metabolomics and Molecular Phylogenetics of Melon (Cucumis melo, Cucurbitaceae) Biodiversity. Metabolites, 10(3), 121.
Corresponding Organization : Agricultural Research Organization
Other organizations :
Biologie du Fruit et Pathologie, Université de Bordeaux, Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement, MetaboHUB, James Hutton Institute, Weizmann Institute of Science, Rothamsted Research, Universidad Nacional de Córdoba, Centro Científico Tecnológico - Córdoba, Max Planck Institute of Molecular Plant Physiology, University of Liverpool, University of Copenhagen, Wageningen University & Research, Graduate School Experimental Plant Sciences
Leaf tissue from 44 of the 51 accessions listed in Table 1
Total genomic DNA isolation method described by Gur et al. [37]
DNA quality analysis using ND-1000 spectrophotometer and agarose gel electrophoresis
DNA concentration estimation using Qubit® 2.0 Fluorometer and Qubit® dsDNA BR Assay Kit
controls
Positive control: Not mentioned
Negative control: Not mentioned
Annotations
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