Samples and a mixture of 240 standards (in three mixes) were separated by HPLC on a Dionex Ultimate 3000 RSLC system (Thermo Fisher Scientific) using a ZIC-pHILIC (Merck) column and then analyzed on a Q Exactive Orbitrap mass spectrometer (Thermo Fisher Scientific) using the method of Rattigan et al. (93 (link)), including fragmentation of the pooled samples.
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