Mastomys coucha from the DKFZ colonies were maintained under SFP conditions in individually ventilated cages (Tecniplast GR900) or in type 3 cages in positive pressure isolators on aspen bedding with curled wood wool as environmental enrichment. Mastomys were constantly kept in a light/dark cycle of 14/10h, an average temperature of 22+/-2°C and a humidity of 55+/-10% according to Directive 2010/63/EU, appendix III and the German legislation. Mastomys were fed ad libitum (Mouse and Rat Maintenance No. 3437, KLIBA NAFAG, Kaiseraugst, Switzerland) and had unlimited access to autoclaved water.
Virus-free animals were obtained by hysterectomies of pregnant Mastomys coucha under sterile conditions and kept in a specific pathogen free isolator unit with positive air pressure at the DKFZ [21 (link)]. To confirm the virus-free status of the animals, sera and skin samples are regularly tested by ELISA (E2 and L1) and PCR, respectively.
Virus-free animals were obtained by hysterectomies of pregnant Mastomys coucha under sterile conditions and kept in a specific pathogen free isolator unit with positive air pressure at the DKFZ [21 (link)]. To confirm the virus-free status of the animals, sera and skin samples are regularly tested by ELISA (E2 and L1) and PCR, respectively.
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