Plasmid Transfection and Mitochondrial Dynamics

The following plasmids have been used in the manuscript: human SATB1 (Origene), human GBA (Origene), pCMV6 empty vector (Origene), human a‐SYN (A53T mutant) (Addgene), and mt‐Keima (Addgene). The alphasynuclein‐A53T plasmid was a gift from David Rubinsztein (Addgene plasmid # 40823; http://n2t.net/addgene:40823; RRID:Addgene_40,823) (Furlong et al., 2000 (link)); mt‐mKeima was a gift from Richard Youle (Addgene plasmid # 131626; http://n2t.net/addgene:131626; RRID:Addgene_131,626) (Vargas et al., 2019 (link)). mKeima‐Red‐Mito‐7 was a gift from Michael Davidson (Addgene plasmid # 56018; http://n2t.net/addgene:56018; RRID:Addgene_56,018) Transfections have been performed with Lipofectamin 3000 (Thermofisher) according to the manufacturer's protocol.

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Russo T., Kolisnyk B., B. S. A., Plessis‐Belair J., Kim T.W., Martin J., Ni J., Pearson J.A., Park E.J., Sher R.B., Studer L, & Riessland M. (2024). The SATB1‐MIR22‐GBA axis mediates glucocerebroside accumulation inducing a cellular senescence‐like phenotype in dopaminergic neurons. Aging Cell, 23(4), e14077.

Publication 2024

human SATB1 human GBA pCMV6 empty vector mt‐Keima mt‐mKeima Lipofectamin 3000

Corresponding Organization : Stony Brook University

Other organizations : Rockefeller University, Memorial Sloan Kettering Cancer Center, Stony Brook School, Baylor College of Medicine

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Variable analysis

independent variables

Human SATB1
Human GBA
Human a-SYN (A53T mutant)
Mt-Keima

dependent variables

Not explicitly mentioned

control variables

PCMV6 empty vector

controls

Positive control: None specified
Negative control: pCMV6 empty vector

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