The rats were anesthetized and fluorescein isothiocyanate-Dextran (FITC-D) (Sigma-Aldrich Corp, USA) was injected into the tail vein. Retinas and eyecups were isolated and tiled on glass slides, and fluorescence images were captured with a fluorescence microscope (Nikon). The specific operation method is carried out according to the previously reported scheme [20 (link)].
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