Amylolytic Activity Assay Protocol

Ethyl acetate, chloroform, methanol,
ethylene, n-hexane, gallic acid, sodium hydro-oxide,
dimethyl sulfoxide, acarbose, buffer solution, potassium sodium tartrate,
DNS (3,5-dinitrosalicylic acid), amylase (aspergillus), etc. were
used in the experiments. Majority of the reagents were purchased from
Sigma-Alrdich (Steinheim, Germany).

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Ishtiaq H., Ahmad B., Zahid N., Bibi T., Khan I., Azizullah A., Ahmad K., Murshed A., Rehman S.U., Abdel-Maksoud M.A., El-Tayeb M.A., Lu J, & Zaky M.Y. (2024). Phytochemicals, Antioxidant, and Antidiabetic Effects of Ranunculus hirtellus Aerial Parts and Roots: Methanol and Aqueous Extracts. ACS Omega, 9(20), 21805-21821.

Publication 2024

Corresponding Organization : Guangdong Medical College

Other organizations : Abbottabad University of Science and Technology, COMSATS University Islamabad, King Saud University, Beni-Suef University

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Variable analysis

independent variables

Ethyl acetate
Chloroform
Methanol
Ethylene
N-hexane
Gallic acid
Sodium hydro-oxide
Dimethyl sulfoxide
Acarbose
Buffer solution
Potassium sodium tartrate
DNS (3,5-dinitrosalicylic acid)
Amylase (aspergillus)

dependent variables

Measured outcomes

control variables

Kept constant to ensure valid results

controls

Positive controls
Negative controls

Annotations

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