EZH2 Chromatin Immunoprecipitation Protocol

In this study, ChIP experiments were conducted utilizing the Magna ChIP Kit (Millipore, Bedford, MA) under the instruction of the manufacturer [15 (link)]. Cells were treated with 4% formaldehyde and the cell lysate was sonicated to interrupt intact chromatin into chromatin fragments (between 200 and 300 bp). Then, chromatin was immunoprecipitated with anti-EZH2 antibody from Abcam, and IgG was treated as a control. The precipitated chromatin DNA was recovered and measured using qRT-PCR. Among them, the primers used for ChIP-PCR are shown in Additional file 2: Table S1.

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Chen Z., Tang W., Zhou Y, & He Z. (2022). The role of LINC01419 in regulating the cell stemness in lung adenocarcinoma through recruiting EZH2 and regulating FBP1 expression. Biology Direct, 17, 23.

Publication 2022

Magna ChIP Kit anti-EZH2 antibody

Anti antibody Cell Chip Chromatin Ezh2 Formaldehyde Primers

Corresponding Organization :

Other organizations : Sir Run Run Shaw Hospital, Zhejiang University

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Variable analysis

independent variables

Treatment of cells with 4% formaldehyde

dependent variables

Chromatin fragment size (between 200 and 300 bp)
Enrichment of chromatin fragments immunoprecipitated with anti-EZH2 antibody compared to IgG control

control variables

Cells used in the study
Magna ChIP Kit (Millipore) used for chromatin immunoprecipitation
Sonication conditions to interrupt chromatin into fragments

controls

Not specified

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