Assessing Cell Viability with MET and PF 05175157

The MET (PHR1084, Sigma-Aldrich, Laramie, WY, USA) stock solution was prepared in standard NB cell culture medium. The acetyl CoA carboxylase (ACC) inhibitor PF 05175157 (5790, Tocris, Bristol, UK) was dissolved in DMSO and stored at −80 °C. 1.9 × 10⁴ cells/well of SKNBE(2) cells and 8 × 10³ cells/well of KELLY cells were seeded in 96-well plates. Then, the cells were treated with different concentrations of MET (1 to 20 mM) for 72 h, or with PF 05175157 (3–33 µM) for 48 h. Standard medium was used as a vehicle control of the MET-treated cells, whereas DMSO was used as a vehicle control of the PF 05175157-treated cells. Cell viability was measured via a crystal violet assay, as previously described [28 (link)].