U87 cells were incubated in each condition for 72 h in 5% CO2 at 37°C. Immunofluorescence was carried out according to established techniques.30 (link) U87 cells were fixed with 4% paraformaldehyde phosphate buffer solution (Nacalai Tesque) for 10 min and permeabilized in 0.2% Triton X-100/PBS for 5 min. Samples were blocked with Blocking One (Nacalai Tesque), and the primary antibody was diluted with Can Get Signal® (Toyobo).
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