The following ingredients make up the basal broth medium (BM) for the yield of levan: sucrose (80), yeast extract (1.0), K2HPO4 (1.0), and MgSO4 (0.2)21 . The chosen isolates were each given a freshly made culture that was placed separately into a 250 mL Erlenmeyer flask. Each flask held 50 mL of the sample (BM).
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