Photon Irradiation Protocol for In Vitro Studies

Photon irradiations were carried out at the medical linear accelerator (LINAC) of Virgen de las Nieves Hospital (HVN) in Granada. The Elekta Versa HD™ accelerator delivered a flattened 6 MV high energy photon beam [19 (link)]. The irradiation zone was adapted in order to carry out in vitro irradiations. The electronic equilibrium was secured by immersing the flasks in distilled water and placing under these 14 cm of solid water (see Figure 3). Two flasks were simultaneously irradiated at room temperature (23–25 °C), both within the field of the beam, and then receiving the same dose. Irradiations of 0.5–6 Gy were performed at 1 Gy/min. dose rate.

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Pedrosa-Rivera M., Praena J., Porras I., Sabariego M.P., Köster U., Haertlein M., Forsyth V.T., Ramírez J.C., Jover C., Jimena D., Osorio J.L., Álvarez P., Ruiz-Ruiz C, & Ruiz-Magaña M.J. (2020). Thermal Neutron Relative Biological Effectiveness Factors for Boron Neutron Capture Therapy from In Vitro Irradiations. Cells, 9(10), 2144.

Publication 2020

Versa HD™ accelerator

Linear accelerator

Corresponding Organization : Universidad de Granada

Other organizations : Institut Laue-Langevin, Institut de Biologie Structurale, Keele University, Hospital Universitario Virgen de las Nieves

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Variable analysis

independent variables

Irradiation dose (0.5-6 Gy)

dependent variables

Unknown - not explicitly mentioned

control variables

Irradiation dose rate (1 Gy/min)
Temperature (23-25 °C)
Solid water thickness (14 cm)
Flasks were immersed in distilled water to ensure electronic equilibrium

controls

Positive control: Not mentioned
Negative control: Not mentioned

Annotations

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