To evaluate the local inflammatory response, structural tissue changes of the muscle fibers and the activity of our extract, seven-micrometer-thick sections stained with hematoxylin and eosin (H&E) Bio-Optica, Milano, Italy). Histological estimations were made as previously described [19 (link)].
Each image was inspected with an optical microscope (Axostar Plus equipped with Axio-Cam MRc, Zeiss, Oberkochen, Germany). The histological results were shown at 10× (100 μm of the Bar scale). The degree of paw damage was evaluated according to a six-point score: 0 = no inflammation, 1 = mild inflammation, 2 = mild/moderate inflammation, 3 = moderate inflammation, 4 = moderate/severe inflammation and 5 = severe inflammation as reported by Petrosino et al. [20 (link)].
Each image was inspected with an optical microscope (Axostar Plus equipped with Axio-Cam MRc, Zeiss, Oberkochen, Germany). The histological results were shown at 10× (100 μm of the Bar scale). The degree of paw damage was evaluated according to a six-point score: 0 = no inflammation, 1 = mild inflammation, 2 = mild/moderate inflammation, 3 = moderate inflammation, 4 = moderate/severe inflammation and 5 = severe inflammation as reported by Petrosino et al. [20 (link)].
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