Ultrastructural Analysis of Cerebral Cortex

Cerebral cortex tissues were fixed in 2.5% glutaraldehyde for 8 h and rinsed with PBS. Secondary fixation was performed using 1% osmic acid solution for at least 1 h. The tissues underwent a graded dehydration series using ethanol (30%, 50%, and 70% uranyl acetate and 80%, 95%, and 100% uranyl acetate). Following dehydration, the samples were embedded in epoxy resin, cut into ultrathin sections, and stained with uranyl acetate and lead citrate. Images were captured using a transmission electron microscope (TEM) (#JEM1400, Japan).

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Zhao J., Chen C., Ge L., Jiang Z., Hu Z, & Yin L. (2024). TAK1 inhibition mitigates intracerebral hemorrhage-induced brain injury through reduction of oxidative stress and neuronal pyroptosis via the NRF2 signaling pathway. Frontiers in Immunology, 15, 1386780.

Publication 2024

Corresponding Organization : Central South University

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Variable analysis

independent variables

Fixation time (8 h) in 2.5% glutaraldehyde
Fixation time (at least 1 h) in 1% osmic acid solution

dependent variables

Ultrastructural morphology of cerebral cortex tissues observed using transmission electron microscopy (TEM)

control variables

PBS rinsing after initial glutaraldehyde fixation
Graded dehydration series using ethanol (30%, 50%, 70%, 80%, 95%, and 100%)
Embedding in epoxy resin
Cutting of ultrathin sections
Staining with uranyl acetate and lead citrate

controls

No positive or negative controls were explicitly mentioned in the provided information.

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