Solid-state NMR Analysis of 13C/15N-Labeled Schizophyllum

S. commune was grown on a PC membrane in medium containing ¹³C labelled glucose and ¹⁵N labelled (NH4)₂SO₄ and treated as described above. All ssNMR experiments were carried out on a 700 MHz Bruker Avance III or an 800 MHz Bruker Biospin spectrometer. Rigid molecules were probed using dipolar-based transfer schemes by combining cross-polarization (CP) with PARIS (Weingarth et al., 2010 ) or SPC5 (Hohwy et al., 1999 ) recoupling schemes. Two-dimensional dipolar-based (NCA-type (Baldus, 2002 )) correlation experiments involved SPECIFIC-CP (Baldus et al., 1998 ) to transfer polarization from ¹⁵N to ¹³C nuclei.
Mobile compounds were probed using J-based INEPT (Morris and Freeman, 1979 ) and TOBSY (Baldus and Meier, 1996 )- transfers as shown elsewhere (Andronesi et al., 2005 (link)). In addition, direct excitation spectra were recorded in order to excite all carbons present in the sample irrespective of molecular motion (see, e.g. Ref. (Labokha et al., 2013 (link))). For further details see Methods S4, for measuring conditions see Tables S1 & S2.