In Vivo Substrate Transport Assays

In vivo transport assays were performed as previously described [62 (link)]. The radioactive substrates used were L-(¹⁴C(U))-phenylalanine, L-(¹⁴C(U))-lysine and L-(methyl-³H)-methionine (PerkinElmer, USA) and L-(¹⁴C(U))-alanine (Amersham Biosciences, Little Chalfont, Buckinghamshire, UK). Transport was assayed at the following concentrations: 50 μM lysine with and without 100 mM of histidine or ornithine, 500 μM alanine or methionine, and 2.5 mM phenylalanine.

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Ruiz S.J., van ’t Klooster J.S., Bianchi F, & Poolman B. (2020). Growth Inhibition by Amino Acids in Saccharomyces cerevisiae. Microorganisms, 9(1), 7.

Publication 2020

))-phenylalanine L-(methyl-3H)-methionine

Alanine Histidine Lysine Methionine Ornithine Phenylalanine Radioactive

Corresponding Organization : University of Groningen

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Variable analysis

independent variables

Concentration of lysine (50 μM)
Presence of histidine (100 mM) or ornithine (100 mM) in addition to lysine
Concentration of alanine (500 μM)
Concentration of methionine (500 μM)
Concentration of phenylalanine (2.5 mM)

dependent variables

Transport of L-(14C(U))-phenylalanine
Transport of L-(14C(U))-lysine
Transport of L-(methyl-3H)-methionine
Transport of L-(14C(U))-alanine

control variables

Previously described in vivo transport assay protocol [62]

controls

No positive or negative controls were explicitly mentioned in the information provided.

Annotations

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