Chromosomal Mapping of Porcine Genome

The breakpoint position was determined by FISH using Bacterial Artificial Chromosome (BAC) clones obtained from the INRAE @BRIDGe Platform (http://abridge.inra.fr/index.php, 1 April 2020) [27 (link)]. The BACs SBAB-91F6 (located on SSC1) and CH242-4A8 (located on SSC15) were labeled with biotin using the BioPrime DNA Labeling System kit (LIFE TECHNOLOGIES, Carlsbad, CA, USA) and revealed by Alexa 594 conjugated to Streptavidin (MOLECULAR PROBES; Eugene, OR, USA). The BAC CH242-29P21 (SSC15) was labeled by digoxygenin and revealed using FITC conjugated mouse anti-digoxygenin antibodies (Sigma, St. Louis, MO, USA), as previously described [31 (link)].

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Mary N., Calgaro A., Barasc H., Bonnet N., Ferchaud S., Raymond-Letron I., Ducos A, & Pinton A. (2021). Meiotic Silencing in Pigs: A Case Study in a Translocated Azoospermic Boar. Genes, 12(8), 1137.

Publication 2021

BioPrime DNA Labeling System kit Alexa 594 conjugated to Streptavidin FITC conjugated mouse anti-digoxygenin antibodies

Alexa 594 Anti antibodies Bacterial artificial chromosome Biotin Clones Fish Fitc Molecular probes Mouse Streptavidin

Corresponding Organization :

Other organizations : Génétique Physiologie et Systèmes d'Elevage, Université de Toulouse, École Nationale Vétérinaire de Toulouse, Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement, Inserm, Université Toulouse III - Paul Sabatier, Centre National de la Recherche Scientifique

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Variable analysis

independent variables

FISH using Bacterial Artificial Chromosome (BAC) clones

dependent variables

Breakpoint position

control variables

BAC clones SBAB-91F6 (located on SSC1) and CH242-4A8 (located on SSC15) labeled with biotin and revealed by Alexa 594 conjugated to Streptavidin
BAC CH242-29P21 (SSC15) labeled by digoxygenin and revealed using FITC conjugated mouse anti-digoxygenin antibodies

controls

Positive control: BAC clones SBAB-91F6 (located on SSC1) and CH242-4A8 (located on SSC15) labeled with biotin and revealed by Alexa 594 conjugated to Streptavidin
Negative control: BAC CH242-29P21 (SSC15) labeled by digoxygenin and revealed using FITC conjugated mouse anti-digoxygenin antibodies

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