Photosensitized Combination Cancer Therapy

HS201, a novel photosensitizer made of VP (Novartis Pharmaceuticals, Basel, Switzerland) tethered to an Hsp90 small molecule inhibitor (HS10), was used for in vitro and in vivo imaging and PDT. HS201 was developed and supplied by Haystead Lab (Department of Pharmacology and Cancer Biology, Duke University) as previously described.23 (link) Anti-PD-L1 antibody (clone; 10F.9G2) was purchased from Bio X cell (New Hampshire USA) and used for animal treatment. Annexin V-Allophycocyanin was purchased from BD Biosciences (California USA) and 7-AAD from Beckman Coulter Inc. (California, USA). Detailed information of antibodies used for flow cytometry is shown in online supplemental methods.

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Kaneko K., Acharya C.R., Nagata H., Yang X., Hartman Z.C., Hobeika A., Hughes P.F., Haystead T.A., Morse M.A., Lyerly H.K, & Osada T. (2022). Combination of a novel heat shock protein 90-targeted photodynamic therapy with PD-1/PD-L1 blockade induces potent systemic antitumor efficacy and abscopal effect against breast cancers. Journal for Immunotherapy of Cancer, 10(9), e004793.

Publication 2022

Anti-PD-L1 antibody (clone; 10F.9G2) Annexin V-Allophycocyanin 7-AAD

7 aad Allophycocyanin Animal Annexin v Anti antibody Antibodies Cancer Cell Clone Flow cytometry Hsp90 Pd l1 Pharmaceuticals Photosensitizer

Corresponding Organization : Duke Medical Center

Other organizations : Duke University

Top 5 similar protocols

Protocol cited in 1 other protocol

Variable analysis

independent variables

HS201 (a novel photosensitizer made of VP tethered to an Hsp90 small molecule inhibitor (HS10))
Anti-PD-L1 antibody (clone; 10F.9G2)

dependent variables

In vitro and in vivo imaging
PDT (Photodynamic Therapy)

control variables

Annexin V-Allophycocyanin
7-AAD

controls

Positive control: Not specified
Negative control: Not specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!