Immunostaining of raft sections was performed as previously described [78 (link)]. To detect MCM7, MCM7 antiserum (sc-65469, Santa Cruz, Dallas, TX) was used at a 1:200 dilution. For BrdU detection, BrdU antiserum (NA-61, Millipore, Burlington, MA) was used at a 1:50 dilution. Staining for p62 and LC3β was carried out using anti-p62 (Abcam) and anti-LC3β (Santa Cruz) antisera, respectively, as previously described [83 (link)].
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