Western blot analysis was performed as described previously 32 (link). Cell Lysates were immunoblotted with purified mouse anti-human Jab1 antibody and β-actin antibody was used as the internal positive control. Finally, the signals were developed with chemiluminescent western blotting substrate (ThermoFisher Scientific), and ImageJ64 software (NIH) was used to quantify signal intensity.
Protocol detail
Quantitative Western Blot Analysis
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Actin
Anti antibody
Antibody
Cell
Human
Mouse
Western blot analysis
Corresponding Organization :
Other organizations : Jiangnan University, The University of Texas MD Anderson Cancer Center, Sun Yat-sen University, Shantou Central Hospital
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Variable analysis
independent variables
- Purified mouse anti-human Jab1 antibody used for immunoblotting
- Signal intensity of Jab1 protein measured using ImageJ64 software
- β-actin antibody used as internal positive control
- β-actin antibody used as internal positive control
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