Investigating [77Br]Br-WC-DZ's Impact on Prostate Cancer Cell Cycle

The effect of [⁷⁷Br]Br-WC-DZ in altering the cell cycle phases of prostate cancer cells PC-3 and IGR-CaP1 was analyzed using a Muse™ Cell Cycle Kit (Luminex, Austin, TX, USA) according to the manufacturer’s instructions. Briefly, after incubating the cells with [⁷⁷Br]Br-WC-DZ (1 nM and 5 nM) for 24 h, the cells were harvested using trypsin and washed with PBS. The cells were then fixed with 70% ethanol kept at −20 °C. The cells were then washed, pelleted, and incubated with Muse cell cycle reagent for 30 min at room temperature in the dark. The percentage of cells in G0/G1, S, and G2/M phases were assessed in Guava Muse™ Cell Analyzer (Luminex, Austin, TX, USA).