Radioligand Binding Assay for Drug Affinity

Conventional competition
and saturation radioligand binding assays were used to determine the
affinities of reference standards and EU93-108. Experiments were carried
out by the NIMH Psychoactive Drug Screening Program (PDSP) and were
performed as previously described.^{123 (link)}The detailed experimental protocols for the radioligand assays are
available on the NIMH PDSP website at https://pdsp.unc.edu/pdspweb/content/UNC-CH%20Protocol%20Book.pdf.

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Harris L.D., Regan M.C., Myers S.J., Nocilla K.A., Akins N.S., Tahirovic Y.A., Wilson L.J., Dingledine R., Furukawa H., Traynelis S.F, & Liotta D.C. (2023). Novel GluN2B-Selective NMDA Receptor Negative Allosteric Modulator Possesses Intrinsic Analgesic Properties and Enhances Analgesia of Morphine in a Rodent Tail Flick Pain Model. ACS Chemical Neuroscience, 14(5), 917-935.

Publication 2023

Psychoactive drug Radioligand assays

Corresponding Organization : Emory University

Other organizations : Cold Spring Harbor Laboratory

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Variable analysis

independent variables

Reference standards
EU93-108

dependent variables

Affinities of reference standards and EU93-108

control variables

Experimental protocols for the radioligand assays

controls

Positive controls
Negative controls

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