Wound Healing Assay with WECP

DPCs were seeded in six-well plates at a density of 1.5 × 10⁵/well and grown overnight to confluence. Wounds were gently induced on the monolayer with a 1,000-μL pipette tip. Cell debris was eliminated. Fresh medium containing 1% (v/v) fetal bovine serum plus various WECP concentrations was added to the wells. The plates were stored in a 5% CO₂ incubator at 37°C overnight. The cells in each plate were pretreated with MK-2206 2HCl for 1 h before the WECP treatments. Identity fields were photographed at each time point, and the healed areas were measured with ImageJ software (National Institutes of Health, Bethesda, MD, United States).