Hesperidin, fluorescein sodium salt, and dimethylsulfoxide (DMSO) were purchased from Sigma‐Aldrich (St. Louis, MO, USA). Dulbecco's modified Eagle's medium (DMEM) and fetal bovine serum (FBS) were obtained from Hyclone Laboratories Inc (Logan, UT, USA). Penicillin–Streptomycin, MEM Non‐Essential Amino Acids (NEAA), and 0.25% Trypsin/EDTA were purchased from GIBCO (Gaithersburg, MD, USA). MITO+™ serum extender, basal medium for seeding, and enterocyte differentiation medium for Caco‐2 cell monolayer cultures were purchased from BD Biosciences (San Jose, CA, USA). Other reagents were analytical grade and used without further purification.
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