Transient Expression of BrERF2 and BrERF109 in Tobacco

The coding sequences (CDS) of BrERF2 and BrERF109 full-length without the stop codon were amplified and fused with the pCAMBIA1300 vector. The recombinant plasmids (35S:BrERF2-GFP and 35S:BrERF109-GFP) or the control vector (35S: GFP), and nuclear marker NLS-RFP (NLS, nuclear localization sequence) were co-transformed into Agrobacterium. Then, the Agrobacterium containing 35S:BrERF2-GFP, 35S:BrERF109-GFP or 35S: GFP was injected into the suction of 4- to 6-week-old tobacco (Nicotiana benthamiana) blade with a 1 mL syringe without needle. The GFP fluorescence was observed by Axioskop 2 Plus fluorescence microscope (Zeiss, Oberkochen, Germany) at 48 h of infiltration [20 (link)].

Free full text: Click here

Yue L., Kang Y., Zhong M., Kang D., Zhao P., Chai X, & Yang X. (2023). Melatonin Delays Postharvest Senescence through Suppressing the Inhibition of BrERF2/BrERF109 on Flavonoid Biosynthesis in Flowering Chinese Cabbage. International Journal of Molecular Sciences, 24(3), 2933.

Publication 2023

Axioskop 2 Plus

Agrobacterium Blade 1 Coding sequences Fluorescence Fluorescence microscope Needle Nicotiana Plasmids Stop codon Suction Syringe Vector

Corresponding Organization : South China Agricultural University

Top 5 similar protocols

Variable analysis

independent variables

Expression of BrERF2-GFP fusion protein
Expression of BrERF109-GFP fusion protein

dependent variables

GFP fluorescence

control variables

Tissue type (tobacco leaves)
Agrobacterium-mediated transformation
Leaf age (4- to 6-week-old tobacco plants)
Observation time (48 hours after infiltration)

controls

Positive control: 35S:GFP (expression of GFP alone)
Nuclear marker: NLS-RFP (nuclear localization sequence)

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!