Overexpression of Rat PRDX2 in ADSCs

Lentivirus expressing rat PRDX2 was purchased from Hanbio Biotechnology (Shanghai, China) by using HBLV-r-PRDX2-3xflag-ZsGreen-PURO. The PRDX2 lentivirus was transfected into passage 2 ADSCs at a multiplicity of infection (MOI) of 100. Puromycin (2 μg/mL) was used to screen ADSCs transfected with PRDX2. After 3 days of screening, we observed green fluorescence by fluorescence microscopy, which indicated that PRDX2 was successfully transfected into ADSCs. In addition, western blotting was performed to detect the expression of PRDX2. After identification, the cells were passaged for subsequent experiments.