The following vaccine strains were purchased from Huapai Bioengineering Group Co. Ltd. (Chengdu, China) and other companies: Ch-1R strain of C-PRRSV, TJM92 strain of HP-PRRSV, C strain of CSFV, O/Mya98/XJ/2010 strain of foot-and-mouth disease virus (FMDV), ZJ/C strain of porcine circovirus type 2 (PCV2), Bartha-K61 strain of porcine pseudorabies virus (PRV), SC1 strain of porcine parvovirus (PPV), and TJ strain of swine influenza virus (SIV). The positive clinical samples of NL-PRRSV, ASFV, and Senecavirus A (SVA) were obtained by our laboratory and confirmed by genomic sequencing. They were stored at −70 °C until used.
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