Transmission Electron Microscopy of Sarkosyl-Insoluble Protein Aggregates

The Sarkosyl insoluble pellets were imaged via transmission electron microscopy (TEM) as previously described [48 (link)]. Briefly, a pellet was pipetted onto carbon-coated TEM grids and incubated for 5 min at room temperature. The grids were then negatively stained with 2% uranyl acetate for 2 min. Samples were imaged using a Hitachi H7500 (Hitachi, Tokyo, Japan) transmission electron microscope equipped with an Advanced Microscopy Sciences XR60 camera (Advanced Microscopy Techniques, Woburn, MA, USA).

Free full text: Click here

Monge F.A., Fanni A.M., Donabedian P.L., Hulse J., Maphis N.M., Jiang S., Donaldson T.N., Clark B.J., Whitten D.G., Bhaskar K, & Chi E.Y. (2023). Selective In Vitro and Ex Vivo Staining of Brain Neurofibrillary Tangles and Amyloid Plaques by Novel Ethylene Ethynylene-Based Optical Sensors. Biosensors, 13(2), 151.

Publication 2023

H7500 XR60 camera

Carbon Microscopy Pellets Sarkosyl Transmission electron microscope Uranyl acetate

Corresponding Organization : University of New Mexico

Other organizations : Sartorius (United States)

Top 5 similar protocols

Variable analysis

independent variables

Sarkosyl insoluble pellets

dependent variables

Transmission electron microscopy (TEM) images of the Sarkosyl insoluble pellets

control variables

Carbon-coated TEM grids
Negative staining with 2% uranyl acetate
Hitachi H7500 transmission electron microscope
Advanced Microscopy Sciences XR60 camera

controls

No positive or negative controls were explicitly mentioned in the provided information.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!