Protocol detail

Drosophila Genetics and Imaging Protocol

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All flies were raised at 25 °C with 12 h/12 h light/dark cycle unless noted. This study: htl-LexA, pyr-Gal4/CyO, htl>FRT>stop>FRT>Gal4, UAS-Pyr:GFP, UAS-Ths:GFP and LexO-Htl:mCherry. All new transgenic injections were performed by Rainbow Transgenic Flies, Inc. Bloomington Drosophila Stock Center: UAS-CD8:GFP, UAS-CD8:RFP, UAS-mCherryCAAX, LexO-CD2:GFP, UAS-Eb1:GFP, UAS-Lifeact:GFP, UAS-nls:GFP, UAS-nls:mCherry, htl-Gal4, ths-Gal4/CyO, UAS-Dia:GFP, UAS-ΔDAD-Dia:GFP, UAS-pyrRNAi, UAS-diaRNAi, hs-Flp, {nos-Cas9}ZH-2A, and w¹¹¹⁸. Vienna Drosophila Resource Center: htl:GFP^fTRG, UAS-htlRNAi, and UAS-thsRNAi. Other sources: LexO-nsyb:GFP^1–10, UAS-CD4:GFP¹¹^{20 (link)}. LexO-mCherryCAAX^{15 (link)}. dpp-Gal4/CyO, LexO-Fz:mCherry and 1151-Gal4 from Huang et al.^{36 (link)} (also see Supplementary Table 3).

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Patel A., Wu Y., Han X., Su Y., Maugel T., Shroff H, & Roy S. (2022). Cytonemes coordinate asymmetric signaling and organization in the Drosophila muscle progenitor niche. Nature Communications, 13, 1185.

Publication 2022

UAS-CD8:GFP UAS-CD8:RFP UAS-mCherryCAAX UAS-Lifeact:GFP UAS-nls:GFP UAS-nls:mCherry htl-Gal4

Drosophila Flies Transgenic

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Variable analysis

independent variables

htl-LexA
pyr-Gal4/CyO
htl>FRT>stop>FRT>Gal4
UAS-Pyr:GFP
UAS-Ths:GFP
LexO-Htl:mCherry
UAS-CD8:GFP
UAS-CD8:RFP
UAS-mCherryCAAX
LexO-CD2:GFP
UAS-Eb1:GFP
UAS-Lifeact:GFP
UAS-nls:GFP
UAS-nls:mCherry
htl-Gal4
ths-Gal4/CyO
UAS-Dia:GFP
UAS-ΔDAD-Dia:GFP
UAS-pyrRNAi
UAS-diaRNAi
hs-Flp
{nos-Cas9}ZH-2A
htl:GFP^fTRG
UAS-htlRNAi
UAS-thsRNAi
LexO-nsyb:GFP^1–10
UAS-CD4:GFP¹¹²⁰
LexO-mCherryCAAX¹⁵
dpp-Gal4/CyO
LexO-Fz:mCherry
1151-Gal4

dependent variables

Not explicitly mentioned

control variables

All flies were raised at 25 °C with 12 h/12 h light/dark cycle unless noted
w¹¹¹⁸

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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