Porcine kidney epithelial PK-15 (CCL-33, ATCC), porcine alveolar macrophages 3D4/21 (CRL-2843, ATCC), and human cervical cancer HeLa (CL-82, ATCC) cells were grown in monolayers at 37 °C under 5% CO2 in DMEM (Gibco, Waltham, MA, USA) supplemented with 10% FBS (Gibco), 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate (Sangon, Shanghai, China). Virus titers were determined by the 50% tissue culture infective dose (TCID50) assay, which was calculated with the Reed–Muench method.
The virulent PRV isolate QXX (PRV-QXX) was kindly donated by Yong-Tao Li from the College of Veterinary Medicine, Henan Agricultural University [20 (link)]. The recombinant PRV strain of PRV-GFP, derived from the PRV Hubei strain with the TK gene replaced by a GFP expression cassette from the pEGFP-N1 plasmid, was kindly donated by Han-Zhong Wang from Wuhan Institute of Virology, Chinese Academy of Sciences [21 (link)].
Full-length porcine AP2B1 cDNA was cloned into the mCherry-N1 expression plasmid using the BamHI and KpnI restriction sites.
The virulent PRV isolate QXX (PRV-QXX) was kindly donated by Yong-Tao Li from the College of Veterinary Medicine, Henan Agricultural University [20 (link)]. The recombinant PRV strain of PRV-GFP, derived from the PRV Hubei strain with the TK gene replaced by a GFP expression cassette from the pEGFP-N1 plasmid, was kindly donated by Han-Zhong Wang from Wuhan Institute of Virology, Chinese Academy of Sciences [21 (link)].
Full-length porcine AP2B1 cDNA was cloned into the mCherry-N1 expression plasmid using the BamHI and KpnI restriction sites.
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