Immunohistochemistry of Steroidogenic Enzymes

Formalin-fixed paraffin-embedded tissue blocks were available from all but one patient (Case 10, APA-Y182). Immunohistochemistry (IHC) was performed using specific antibodies against CYP11B2 (mouse monoclonal, Millipore, #MABS1251) (Gomez-Sanchez, et al. 2014 (link)), 11β-hydroxylase (CYP11B1, rat monoclonal) (Gomez-Sanchez, et al. 2017 (link)), 17α-hydroxylase (CYP17A1, mouse monoclonal) (Dharia, et al. 2004 (link)), 3β-hydroxysteroiddehydrogenase/Δ4–5 isomerase type II (HSD3B2, mouse monoclonal) (Gomez-Sanchez et al. 2017 (link)), and β-catenin (mouse monoclonal, BD Biosciences, #610153) essentially as described previously (Monticone, et al. 2012 (link); Nanba et al. 2016 (link)).

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Lerario A.M., Nanba K., Blinder A.R., Suematsu S., Omura M., Nishikawa T., Giordano T.J., Rainey W.E, & Else T. (2019). Genetics of aldosterone-producing adenomas with pathogenic KCNJ5 variants. Endocrine-related cancer, 26(4), 463-470.

Publication 2019

MABS1251

Antibodies Cyp11b1 Cyp17a1 Formalin Hsd3b2 Hydroxylase Immunohistochemistry Isomerase Mouse Paraffin Patient Tissue β catenin

Corresponding Organization : University of Michigan–Ann Arbor

Other organizations : Yokohama Rosai Hospital, Michigan Center for Translational Pathology

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Variable analysis

independent variables

Antibodies used for immunohistochemistry (IHC): CYP11B2 (mouse monoclonal), 11β-hydroxylase (CYP11B1, rat monoclonal), 17α-hydroxylase (CYP17A1, mouse monoclonal), 3β-hydroxysteroiddehydrogenase/Δ4–5 isomerase type II (HSD3B2, mouse monoclonal), and β-catenin (mouse monoclonal)

dependent variables

Expression of the above-mentioned proteins in formalin-fixed paraffin-embedded tissue blocks from patients

control variables

Formalin-fixed paraffin-embedded tissue blocks were available from all but one patient (Case 10, APA-Y182)
Immunohistochemistry (IHC) was performed using specific antibodies as described in the references provided

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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