Immunofluorescence Staining of WTAP and BCL6 in OCI-Ly10 Cells

After cultures with PU-H71 or DMSO for 48 h, OCI-Ly10 cells were washed twice in PBS, spread onto slides, air dried and fixed in acetone at − 20 °C for 15 min. Immunofluorescence staining was performed as described previously [13 (link)]. After treatment of 0.3% Triton X-100 (KeyGen Biotech) for 10 min, the slides were blocked with Goat serum (Boster Biological Technology) for 1 h at room temperature. The cells were then incubated with the primary antibody against WTAP (diluted 1:50, Santa Cruz, sc-374280) and BCL6 (diluted 1:100, Abcam, ab33901) overnight at 4 °C. Signals were detected with Alexa Fluor 488 and Alexa Fluor 594, respectively. Nuclei in live cells were stained with 4', 6-diamidino-2-phenylindole (DAPI), and visualized with laser confocal microscopy (NikonA1R).