Measuring Cellular Oxidative Stress

Single-cell suspensions were acquired from the liver tissues and prepared to measure cellular ROS 2ʹ,7ʹ-dichlorofluorescein diacetate (DCFDA) in all groups by flow cytometry as described previously.23 (link) The samples were acquired on the MACSQuant Analyzer 10 and analyzed using FlowJo software v10.7.

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Mousa A.M., Soliman K.E., Alhumaydhi F., Almatroudi A., Al Rugaie O., Allemailem K.S., Alrumaihi F., Khan A., Rezk M.Y., Aljasir M., Alwashmi A.S., Aba Alkhayl F.F., Albutti A.S, & Seleem H.S. (2021). Garlic Extract Alleviates Trastuzumab-Induced Hepatotoxicity in Rats Through Its Antioxidant, Anti-Inflammatory, and Antihyperlipidemic Effects. Journal of Inflammation Research, 14, 6305-6316.

Publication 2021

MACSQuant Analyzer 10

Cellular Flow cytometry Liver Tissues

Corresponding Organization : Qassim University

Other organizations : Benha University, Sohag University, Zagazig University, Menoufia University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Cellular ROS (reactive oxygen species) measured by 2',7'-dichlorofluorescein diacetate (DCFDA) using flow cytometry

control variables

Liver tissues used to obtain single-cell suspensions
DCFDA assay and flow cytometry protocol as described in the referenced publication

controls

No positive or negative controls were explicitly mentioned in the provided information

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