Protocol detail

Bovine PMN Confrontation with Toxoplasma

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Toxoplasma gondii (RH strain) tachyzoites were maintained by serial passages in MARC-145 (Meat Animal Research Center-145) layers. The infection rate in MARC was 40-50% in all experiments. Therefore, free-released T. gondii tachyzoites were harvested from MARC supernatants, pelleted (400 × g, 12 min), counted in a Neubauer chamber, suspended in HBSS and used for bovine PMN confrontation. All experiments were performed at an MOI of 1:2 (bPMN:parasites).

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Velásquez Z.D., Peixoto R., Gärtner U., Hermosilla C., Taubert A, & Conejeros I. (2023). Dynamics of cell cycle proteins involved in Toxoplasma gondii-induced bovine NET formation. Frontiers in Immunology, 14, 1125667.

Publication 2023

Bovine Hbss Infection Marc Meat Parasites Strain Toxoplasma gondii

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Variable analysis

independent variables

MOI (Multiplicity of Infection) of 1:2 (bPMN:parasites)

dependent variables

Infection rate in MARC-145 layers

control variables

Toxoplasma gondii (RH strain) tachyzoites were maintained by serial passages in MARC-145 layers
The infection rate in MARC was 40-50% in all experiments
Free-released Toxoplasma gondii tachyzoites were harvested from MARC supernatants, pelleted (400 × g, 12 min), counted in a Neubauer chamber, suspended in HBSS and used for bovine PMN confrontation

controls

Positive control: Not mentioned
Negative control: Not mentioned

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