Glutathione-Mediated Detoxification Assay

1-chloro-2, 4-dinitrobenzene (CDNB), phenylmethylsulfonylfluoride (PMSF), reduced glutathione (GSH), glutathione sepharose 4 fast flow, sephacryl S-300, cumene hydroperoxide, p-hydroxymercuribenzoate, lithocholic acid, hematin, p-chloromercuribenzoic acid (pCMB), N-p-tosyl-l-phenylalanine chloromethyl ketone (TPCK), iodoacetamide, gel filtration molecular weight markers, and triphenyltin chloride were purchased from Sigma Chemical Co. All other chemicals were of analytical grade.

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Masoud H.M., Helmy M.S., Darwish D.A, & Ibrahim M.A. (2023). Purification, characterization, and enzyme kinetics of a glutathione S transferase from larvae of the camel tick Hyalomma dromedarii. Journal of Genetic Engineering & Biotechnology, 21, 28.

Publication 2023

1-chloro-2, 4-dinitrobenzene phenylmethylsulfonylfluoride reduced glutathione sephacryl S-300 cumene hydroperoxide lithocholic acid hematin p-chloromercuribenzoic acid (pCMB), N-p-tosyl-l-phenylalanine chloromethyl ketone (TPCK), iodoacetamide gel filtration molecular weight markers triphenyltin chloride

Cumene hydroperoxide Dinitrobenzene Gel filtration Glutathione Hematin Iodoacetamide Ketone Lithocholic acid Molecular markers P chloromercuribenzoic acid P hydroxymercuribenzoate Phenylalanine Reduced glutathione Sepharose Triphenyltin chloride

Corresponding Organization : National Research Centre

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Variable analysis

independent variables

1-chloro-2, 4-dinitrobenzene (CDNB)
Phenylmethylsulfonylfluoride (PMSF)
Reduced glutathione (GSH)
Glutathione sepharose 4 fast flow
Sephacryl S-300
Cumene hydroperoxide
P-hydroxymercuribenzoate
Lithocholic acid
Hematin
P-chloromercuribenzoic acid (pCMB)
N-p-tosyl-l-phenylalanine chloromethyl ketone (TPCK)
Iodoacetamide
Gel filtration molecular weight markers
Triphenyltin chloride

dependent variables

Measured outcomes not explicitly mentioned

control variables

All other chemicals were of analytical grade

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